Testing of an Antitumor Enzyme L-lysine-A-oxidase from Trichoderma Harzianum Rifai F-180
نویسندگان
چکیده
An enzyme L-lysine-A-oxidase has been obtained from Trichoderma harzianum Rifai F-180. The analysis of serum from CBA mice immunized five times with native L-lysine-A-oxidase intravenously, at a dose of 35 U/kg, showed that the preparation had virtually no effect on the immune response to Tdependent antigen. L-lysine-A-oxidase at a dose of 35 U/kg administered twice intradermally has no effect on the delayed-type hypersensitivity. Multiple, parenteral administration of the native enzyme at doses of 35 U/kg and 70 U/kg slow down the spontaneous migration of leukocytes.
منابع مشابه
Kinetic characteristics of L-lysine α- oxidase from Trichoderma cf. aureoviride Rifai VKM F-4268D: Substrate specificity and allosteric effects
The present work aims to investigate the kinetic characteristics of homodimer enzyme L-lysine α-oxidase from Trichoderma cf. aureoviride Rifai VKM F-4268D, taking into account allosteric effects. The enzyme was first shown to reveal positive cooperativeness, h=2.05±0.15. Using additional opportunities of Hill coefficient the value of the Michaelis-Menten constant has been estimated, Km=1.015∙10...
متن کاملProduction, purification and characterization of D-aspartate oxidase from the fungus Trichoderma harzianum SKW-36
Trichoderma harzianum Rifai SKW-36 produced two kinds of D-amino acid oxidizing enzymes. One enzyme was D-aspartate oxidase acting on acidic D-amino acids such as D-aspartate and D-glutamate and another one was D-amino acid oxidase acting on neutral D-amino acid such as D-phenylalanine and D-methionine. These enzymes in the cell-free extract were separated by DEAE-Toyopearl ion-exchange column ...
متن کاملAntagonistic activity of Penicillium oxalicum Corrie and Thom, Penicillium decumbens Thom and Trichoderma harzianum Rifai isolates against fungi, bacteria and insects in vitro.
The antibiotic activity of 70 isolates belonging to the genera Aspergillus, Penicillium, Fusarium, Alternaria and Trichoderma was tested as preliminary screening. The highest activity was obtained with three Penicillium oxalicum isolates, one Penicillium decumbens isolate and the Trichoderma harzianum isolate. After that, we chose these five isolates in order to carry out other studies with bac...
متن کاملHydrolysis of xylans by enzyme systems from solid cultures of Trichoderma harzianum strains.
Xylanase activity was isolated from crude extracts of Trichoderma harzianum strains C and 4 grown at 28 degree C in a solid medium containing wheat bran as the carbon source. Enzyme activity was demonstrable in the permeate after ultrafiltration of the crude extracts using an Amicon system. The hydrolysis patterns of different xylans and paper pulps by xylanase activity ranged from xylose, xylo...
متن کاملEvaluation of Biological Efficacy of Trichoderma Species Isolates against Alternaria Leaf Spot Disease of Sesame
Alternaria leaf spot disease is a major threat to sesame (Sesamum orientale L.) caused by Alternari asesami. Induced resistance is an alternative to systemic disease resistance response of plants. The present study aims to evaluate Trichoderma species efficacy as biocontrol via induction of resistance against A. sesami in sesame species. During in vitro bio control test, T. harzianum colonize a...
متن کامل